Enrico Stefani, M.D., Ph.D.
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Vice Chair, Research,
Anesthesiology
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Director,
Molecular Medicine
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Professor,
Anesthesiology
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Member,
ACCESS Program: Dept. of Molecular, Cell & Integrative Physiology,
Neuroscience Graduate Program,
NanoBiotechnology and Biomaterials,
Brain Research Institute,
California NanoSystems Institute
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Faculty,
Cardiovascular Research Laboratory
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Education: |
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Degrees:
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Ph.D., University College, University of London, 1969
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M.D.:
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1964 Facultad de Medicina, Universidad de Buenos Aires, Argentina. Honor Degree., Facultad de Medicina, Universidad de Buenos Aires, Argentina. Honor Degree.
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Honors and Awards: |
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2003 University of Colima, Colima, Mexico, Research building named "Unidad de Investigaciones Enrico Stefani"
1998 - 2001 UCLA Council of Academic Promotions, Member
1997 - 2006 Guggenheim Review Committee, Member
1997 - 1998 Argentinean Research Council (CONICET), President
1997 Gordon Conference "EC-Coupling", Session Leader
1997 SAIB meeting, Cordoba, Argentina, Plenary lecture
1997 SOBLA meeting, Buenos Aires, Argentina, Plenary lecture
1996 Gordon Conference "Ion Channels", Vice Chairman
1996 UCLA, Magoun Lecture
1994 Gordon Conference "EC-Coupling", Session Leader
1991 - 1996 The Physiology Study Section, NIH, Regular member
1991 Gordon Conference "Excitation -Contraction Coupling", Chairman
1987 - 1990 Sociedad Latinoamericana de Biofísica, President
1986 - 1987 Sociedad Latinoamericana de Biofísica, Vice President
1985 - 1987 Sistema Nacional de Investigadores. Mexico, National Investigator. Top Level
1981 Academia de la Investigacion Cientifica, A. C. of Mexico, National Award in Natural Sciences
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Certifications: |
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Professional Societies:
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2003 Society of Cell Biology, Society of Cell Biology
2003 Society of Endocrinology, Society of Endocrinology
1990 American Physiological Society, USA, American Physiological Society, USA
1984 New York Academy of Sciences, USA, New York Academy of Sciences, USA
1979 Biophysical Society, USA, Biophysical Society, USA
1975 International Brain Research Organization, International Brain Research Organization
1975 Society for Neuroscience, USA, Society for Neuroscience, USA
1975 Sociedad Mexicana de Ciencias Fisiologicas, Mexico, Sociedad Mexicana de Ciencias Fisiologicas, Mexico
1971 Sociedad Argentina de Biof��sica, Argentina, Sociedad Argentina de Biof��sica, Argentina
1969 Physiological Society, Great Britain, Physiological Society, Great Britain
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Certification Type:
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1976 Academia de la Investigacion Cientifica, Mexico, Academia de la Investigacion Cientifica, Mexico
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Contact Information:
| Work Address: |
Office
CHS
Box 957115
Los Angeles, CA 90095
UNITED STATES
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| Work Address: |
Laboratory
CHS
Los Angeles, CA 90095
UNITED STATES
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Lab Number:
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(310) 825-6649
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Work Phone Number:
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(310) 794-7808
Laboratory
310-794-7804
Office
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Research Interests:
Cell Biology of Ion Channels
Traditionally, my laboratory has been focused to define the molecular determinants of voltage dependent Ca2+ and K+ channel function. More recently, my interest has been directed to investigate the molecular remodeling of macromolecular signaling complexes of K+ and Ca2+ voltage dependent channels in health and disease, in order to unravel new concepts to be applied to Molecular Medicine. We are using as model systems myometrium and heart at different stages of pregnancy. We are investigating: 1. The molecular mechanisms of ion channel regulation by sex hormones and their functional consequences in myometrium and 2. The molecular signature and heart estrogen receptor alpha pathways involved in heart functional hypertrophy that occurs in pregnancy. To perform these investigations, we are using a multidisciplinary approach involving molecular biology, biochemical, immunocytochemical and functional techniques. We are using real time PCR for accurate quantification of transcript levels, proteomics to discover molecular partners, high resolution confocal microscopy with 3D reconstruction for colocalization of various proteins in their native cellular compartments and small animal imaging techniques to follow ?in vivo? changes of heart function. We have developed new statistical methods to quantify the degree of protein-protein proximity by evaluating the intensity landscape correlation among sets of proteins. These studies are performed in conjunction with microarray analysis to investigate changes in global gene expression and as a discovery tool for novel genes involved in ion channel remodeling.
1. Molecular mechanisms and metabolic pathways of K+ channel remodeling in heart functional hypertrophy during pregnancy. During pregnancy, there is an increased risk of arrhythmias and the heart develops functional hypertrophy. We are investigating K+ channel molecular remodeling and metabolic pathways during heart functional hypertrophy that may explain the cardiac risks during pregnancy and their reversibility after delivery. These studies are focused on the molecular constituents of heart transient outward currents (Ito, Kv1.4, Kv4.2 and Kv4.3 genes and regulatory subunits). We recently discovered that transcripts and protein levels of some of the molecular constituents of heart Ito were reduced in late pregnancy. This downregulation can also be mimicked by estrogen treatment in whole animals and in cultured cardiomyocytes. We also discovered that heart myocytes possess membrane estrogen receptors that can be visualized with immunocytochemical methods that may underlie acute actions of estrogen on K+ channel activity. Furthermore, c-Src tyrosine kinase activity, which is activated by estrogen, reduced expression of Kv4.3 resulting in action potential prolongation and Ito current reduction. Thus, we are proposing that estrogen dependent c-Src activity is one of the molecular pathways involved in K+ channel remodeling during functional heart hypertrophy. In addition, molecular genetic studies are performed to investigate the promoter region as a mechanism of direct K channel gene regulation by estrogen (NIH RO1 HL071824).
2. Dynamic changes of protein signaling complexes associated with mitochondria during cardioprotection and ischemic preconditioning. One of the goals is to investigate the signaling kinases/phosphatases cascades and other partner proteins associated with heart mouse mitochondria in control conditions and during ischemia/reperfusion injury. In addition to determining at the biochemical level the activity of a set of signaling molecules, we are investigating their subcellular localization and potential association with mitochondria and changes under ischemia/reperfusion injury. We are using a proteomic approach together with high resolution confocal microscopy to identify dynamic changes of protein signaling complexes associated with mitochondria. High resolution images of heart cells reflect a snapshot of the cardiomyocyte protein organization at the moment of the animal death and heart dissection. Both methods provide independent information on the molecular partners associated to mitochondria function. We have achieved enough spatial resolution to visualize and define the degree of proximity and association between mitochondria and signaling molecules by image restoration in conjunction with the use of analytical algorithms to evaluate the degree of protein-protein proximity. (NIH PPG P01 HL 080111; Project 3 and Mitochondria Biology Core).
3. Mechanisms of hormonal control of ion channels in myometrium. We are using myometrium from animals at different stages of pregnancy and under various hormonal treatments to investigate molecular remodeling of ion channels under the influence of hormones. These studies are focused on Ca2+-activated K+ channels (MaxiK), fast transient K+ channels (Kv4.3) and L-type Ca2+ channels. One of the goals is to unravel the molecular mechanisms involved in the hormonal regulation of ion channels. To this end, we are investigating the role of sex hormones on channel cell biology such as transcriptional regulation, intracellular trafficking and targeting to the cell membrane. We are also using microarray technology in conjunction with functional genomics to identify gene regulation mechanism(s) by hormones. We recently discovered that estrogen downregulates Kv4.3 channel protein levels by transcriptional regulation and that a novel mechanism of hormonal regulation is changes in intracellular trafficking and targeting to the plasma membrane. We also obtained the myometrium transcriptomes before and during parturition with microarray analysis. This new information is analyzed to define protein changes and discover new genes involved in myometrium functional and pathological changes during pregnancy. (RO1 HD046510).
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Additional Information:
Dr. Stefani obtained his MD at the University of Buenos Aires, Argentine and his PhD in Physiology at the Department of Biophysics, University College London under the direction of Dr. R. Miledi FRS and Dr. Sir Bernard Katz, FRS. Dr. Stefani is distinguished by opening new research fields with technical innovations using biophysical approaches. He discovered neuronal serotonin receptors, nerve trophic influence on channel expression, L-type calcium channels in skeletal muscle, and hormonal regulation of ion channels and mechanisms in heart hypertrophy. He pioneered molecular aspects K and Ca channels voltage-sensing mechanisms. Dr Stefani discovered that L-type calcium channels of skeletal muscle as nifedipine receptors can have a role on excitation-contraction coupling. He pioneered studies on voltage dependent ion channel excitability with high resolution recordings in expressed K and Ca channels. He recorded the electrical manifestation of voltage dependent conformation al changes of ion channels referred as gating currents and demonstrated that these precedes pore opening that is also regulated by beta subunits. Recently Dr. Stefani is investigating mechanisms of heart protection during ischemia developing high resolution microscopy techniques beyond the diffraction limit. Dr. Stefani has more than 200 publications in frontier journals
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Helguera, G. Eghbali, M. Sforza, D. Minosyan, T. Y. Toro, L. Stefani, E. , Changes in global gene expression in rat myometrium in transition from late pregnancy to parturition, Physiological Genomics, 2009, 36 ( 2), 89-97.
Vlashi, E. Kim, K. Lagadec, C. Donna, L. D. McDonald, J. T. Eghbali, M. Sayre, J. W. Stefani, E. McBride, W. Pajonk, F. , In Vivo Imaging, Tracking, and Targeting of Cancer Stem Cells, Journal of the National Cancer Institute, 2009, 101 ( 5), 350-359.
Stefani, E. Boffetta, P. Ronco, A. Deneo-Pellegrini, H. Acosta, G. Mendilaharsu, M. , Dietary patterns and risk of bladder cancer: a factor analysis in Uruguay, Cancer Causes & Control, 2008, 19 ( 10), 1243-1249.
Gomes, P. Saito, T. del Corsso, C. Alioua, A. Eghbali, M. Toro, L. Stefani, E. , Identification of a functional interaction between Kv4.3 channels and c-Src tyrosine kinase, Biochimica Et Biophysica Acta-Molecular Cell Research, 2008, 1783 ( 10), 1884-1892.
Alioua, A. Lu, R. Kumar, Y. Eghbali, M. Kundu, P. Toro, L. Stefani, E. , Slo1 caveolin-binding motif, a mechanism of caveolin-1-Slo1 interaction regulating Slo1 surface expression, Journal of Biological Chemistry, 2008, 283 ( 8), 4808-4817.
Kundu, P. Alioua, A. Stefani, E. Toro, L. , A genomic mechanism of estrogen action regulates mouse MaxiK alpha subunit expression from its proximal promoter, Biophysical Journal, 2007, volume (number), 94a-94a.
Minosyan, T. Y. Ciobotaru, A. Toro, L. Stefani, E. Eghbali, M. , Caveolin 3 associates with KCNE2 and ERG1 in adult mouse heart, Biophysical Journal, 2007, volume (number), 126a-126a.
Fishfader, P. Minosyan, T. Y. Eghbali, M. Toro, L. Stefani, E. , Distinct regulation of estrogen receptor alpha isoforms by estrogen, Biophysical Journal, 2007, volume (number), 620a-620a.
Saito, T. Eghbali, M. Vallejo, J. Toro, L. Stefani, E. , Gender regulates the transient outward K+ currents in mouse right ventricular myocytes, Biophysical Journal, 2007, volume (number), 463a-463a.
Minosyan, T. Y. Lu, R. Eghbali, M. Toro, L. Stefani, E. , Increased 5-HT contractile response in late pregnant rat myometrium is associated with a higher density of 5-HT2A receptors, Journal of Physiology-London, 2007, 581 ( 1), 91-97.
Kundu, P. Alioua, A. Stefani, E. Toro, L. , Regulation of Mouse Slo Gene Expression - Multiple promoters, transcription start sites, and genomic action of estrogen, Journal of Biological Chemistry, 2007, 282 ( 37), 27478-27492.
Lu, R. Alioua, A. Kumar, Y. Stefani, E. Toro, L. , Tyrosine phosphorylation motif "pYxx psi" in 5-HT2AR is dispensable for its interaction with c-Src in vitro, Biophysical Journal, 2007, volume (number), 433a-433a.
Vallejo, J. Eghbali, M. Toro, L. Stefani, E. , Altered trafficking of Large Conductance Ca2+-activated K+ (MaxiK) channels in mouse myometrium during late pregnancy, Faseb Journal, 2006, 20 ( 5), A1241-A1242.
Ropero A., Eghbali M., Minosyan, T.Y., Tang, G., Toro L. and Enrico Stefani, Heart Estrogen Receptor Alpha: Distinct Membrane and Nuclear Distribution Patterns and Regulation by Estrogen, Journal of Molecular and Cellular Cardiology, 2006, (In Press).
Eghbali M., Wang Y., Toro L., and Enrico Stefani, Heart hypertrophy during pregnancy: a better functioning heart? Trends in Cardiovascular Medicine, , 2006, (In Press).
Tanaka, Y. Tang, G. H. Takizawa, K. Otsuka, K. Eghbali, M. Song, M. Nishimaru, K. Shigenobu, K. Koike, K. Stefani, E. Toro, L. , K-v channels contribute to nitric oxide- and atrial natriuretic peptide-induced relaxation of a rat conduit artery, Journal of Pharmacology and Experimental Therapeutics, 2006, 317 ( 1), 341-354.
Toro B., Cox N., Wilson R.J., Garrido-Sanabria, E., Enrico Stefani, Toro L., and Zarei M. M., KCNMB1 Regulates Surface Expression of a Voltage and Ca2+-activated K+ Channel via Endocytic Trafficking Signals, Neuroscience, 2006, (In Press).
Tanaka, Y. Tang, G. Takizawa, K. Otsuka, K. Eghbali, M. Song, M. Nishimaru, K. Shigenobu, K. Koike, K. Stefani, E. Toro, L. , Kv channels contribute to nitric oxide- and atrial natriuretic peptide-induced relaxation of a rat conduit artery, J Pharmacol Exp Ther, 2006, 317 (1), 341-54.
Gomes, A.V., Chenggong Zong, Edmondson, R.D., Xiaohai Li, Enrico Stefani, Jun Zhang, Jones R.C., Sheeno Thyparambil, Guang-Wu Wang, Xin Qiao, Fawzia Bardag-Gorce, Peipei Ping, Mapping the Murine Cardiac 26S Proteasome Complexes, Circulation Research , 2006, (Epub ahead of print).
Lu, R. Alioua, A. Kumar, Y. Eghbali, M. Stefani, E. Toro, L. , MaxiK channel partners: physiological impact, Journal of Physiology-London, 2006, 570 ( 1), 65-72.
Rueda, A., Song M., Toro L., Enrico Stefani, and Valdivia H. H., Sorcin Modulation of Ca2+ Sparks in Rat Vascular Smooth Muscle Cells, Journal of Physiology, 2006, (In Press).
Ropero, A. B. Eghbali, M. Deva, R. Toro, L. Stefani, E. , A 43 kDa form of estrogen receptor alpha (ER alpha) associates with membrane structures in rat male hearts, Steroids, 2005, 70 ( 5-7), 480-480.
Zhu, N. Eghbali, M. Helguera, G. Song, M. Stefani, E. Toro, L. , Alternative splicing of Slo channel gene programmed by estrogen, progesterone and pregnancy, FEBS Lett, 2005, 579 ( 21), 4856-60.
Stefani, E. Grabarchuk, V. Eghbali, M. Ropero, A. B. Toro, L. , Discrete protein-protein proximity ("colocalization") analysis in microscopic images, Biophysical Journal, 2005, 88 ( 1), 343a-343a.
Eghbali, M. Deva, R. Alioua, A. Minosyan, T. Y. Ruan, H. M. Wang, Y. B. Toro, L. Stefani, E. , Molecular and functional signature of heart hypertrophy during pregnancy, Circulation Research, 2005, 96 ( 11), 1208-1216.
Eghbali, M. Deva, R. Alioua, A. Wang, Y. B. Toro, L. Stefani, E. , Molecular signature of heart hypertrophy in mice during pregnancy, Biophysical Journal, 2005, 88 ( 1), 119a-119a.
Gomes, P. del Corsso, C. Eghbali, M. Ropero, A. B. Alioua, A. Toro, L. Stefani, E. , Upregulation of Kv4.3 channel activity by c-Src tyrosine kinase dependent phosphorylation, Biophysical Journal, 2005, 88 ( 1), 457a-457a.
Lu, R. Alioua, A. Eghbali, M. Stefani, E. Toro, L. , Activation of c-Src tyrosine kinase as a primary step in 5-HT receptor mediated vasoconstriction is supported by their colocalization in aortic myocytes, Biophysical Journal, 2004, 86 ( 1), 229a-230a.
Zarei, M. M. Eghbali, M. Alioua, A. Song, M. Knaus, H. G. Stefani, E. Toro, L. , An endoplasmic reticulum trafficking signal prevents surface expression of a voltage- and Ca2+-activated K+ channel splice variant, Proceedings of the National Academy of Sciences of the United States of America, 2004, 101 ( 27), 10072-10077.
Alioua, A. Kumar, Y. Lu, R. Eghbali, M. Stefani, E. Toro, L. , Arterial MaxiK, c-Src and 5-HT2A receptors strategically colocalize in lipid rafts and form a macromolecular complex for efficient arterial tone control, Biophysical Journal, 2004, 86 ( 1), 10a-11a.
Alioua, A. Eghbali, M. Minosyan, T. Stefani, E. Toro, L. , Brain MaxiK channel, c-Src and caveolin-1 macromolecular complex: Lipid raft and microvessel localization, Biophysical Journal, 2004, 86 ( 1), 122a-122a.
Virgen, A. Peraza, A. L. Stefani, E. Toro, L. Muniz, J. , Changes in cardiac tissue stiffness associated to functional hypertrophy during pregnancy, Biophysical Journal, 2004, 86 ( 1), 385a-385a.
Garcia-Valdes, J. Alioua, A. Eghbali, M. Stefani, E. Toro, L. , Different pattern of expression and subcellular localization of c-Src, Yes, and Fyn in rat aortic myocytes, Biophysical Journal, 2004, 86 ( 1), 392a-393a.
Pagani, R. Song, M. McEnery, M. Qin, N. Tsien, R. W. Toro, L. Stefani, E. Uchitel, O. D. , Differential expression of alpha 1 and beta subunits of voltage dependent Ca2+ channel at the neuromuscular junction of normal and P/Q Ca2+ channel knockout mouse, Neuroscience, 2004, 123 (1), 75-85.
Alioua, A. Lu, R. Garcia-Valdes, J. Eghbali, M. Stefani, E. Toro, L. , Diminished MaxiK channel expression alters 5-HT-induced contraction in aged rat aorta, Biophysical Journal, 2004, 86 ( 1), 121a-122a.
Ropero, A. B. Deva, R. Eghbali, M. Alioua, A. Toro, L. Stefani, E. , Estrogen receptor alpha localizes in several subcellular compartments in adult rat cardiomyocytes, Biophysical Journal, 2004, 86 ( 1), 50a-51a.
Nishimaru, K. Eghbali, M. Stefani, E. Toro, L. , Function and clustered expression of MaxiK channels in cerebral myocytes remain intact with aging, Exp Gerontol, 2004, 39 (5), 831-9.
Nishimaru, K. Eghbali, M. Rong, L. Marijic, J. Stefani, E. Toro, L. , Functional and molecular evidence of MaxiK channel beta 1 subunit decrease with coronary artery ageing in the rat, Journal of Physiology-London, 2004, 559 ( 3), 849-862.
Deva, R. Eghbali, M. Ropero, A. B. Toro, L. Stefani, E. , Heart transcript levels of estrogen receptor alpha but not beta are under hormonal control, Biophysical Journal, 2004, 86 ( 1), 50a-50a.
Eghbali, M. Toro, L. Stefani, E. , High resolution confocal microscopy and 3D deconvolution reveals that membrane and cytosolic proteins can be organized in distinct cluster patterns, Biophysical Journal, 2004, 86 ( 1), 446a-446a.
Eghbali, M. Toro, L. Stefani, E. , MaxiK channel localizes in dense clusters at the surface of nonpregnant mouse myometrial cells which become undetectable in late pregnancy, Biophysical Journal, 2004, 86 ( 1), 521a-521a.
Toro, L. Alioua, A. Mahajan, A. Nishimaru, K. Zarei, M. M. Stefani, E. , MaxiK, c-Src and vasoconstriction, J Muscle Res Cell Motil, 2004, 25 (8), 616-7.
Stefani, E. Eghbali, M. Minosyan, T. Alioua, A. Toro, L. , Molecular studies in heart hypertrophy during pregnancy, J Muscle Res Cell Motil, 2004, 25 (8), 607.
Mahajan, A. Alioua, A. Stefani, E. Toro, L. , Potassium channel gene expression in smooth muscle during aging, Biophysical Journal, 2004, 86 ( 1), 122a-122a.
Haug, T. Olcese, R. Toro, L. Stefani, E. , Regulation of K+ flow by a ring of negative charges in the outer pore of BKCa channels. Part II: Neutralization of aspartate 292 reduces long channel openings and gating current slow component, J Gen Physiol, 2004, 124 (2), 185-97.
Minosyan, T. V. Eghbali, M. Alioua, A. Toro, L. Stefani, E. , Serotonin receptors (5HT(2A)), caveolin, and c-Src form part of the same macromolecular complex in rat myometrium and are upregulated in late pregnancy, Biophysical Journal, 2004, 86 ( 1), 230a-230a.
Tanaka, Y. Koike, K. Alioua, A. Shigenobu, K. Stefani, E. Toro, L. , beta 1-subunit of MaxiK channel in smooth muscle: a key molecule which tunes muscle mechanical activity, Journal of Pharmacological Sciences, 2004, 94 ( 4), 339-347.
Eghbali, M. Toro, L. Stefani, E. , Diminished surface clustering and increased perinuclear accumulation of large conductance Ca(2+-)activated K+ channel in mouse myometrium with pregnancy, Journal of Biological Chemistry, 2003, 278 ( 46), 45311-45317.
Rueda, A. Alioua, A. Eghbali, M. Song, M. Stefani, E. Toro, L. Valdivia, H. H. , Expression and intracellular localization of sorcin in vascular smooth muscle., Biophysical Journal, 2003, 84 ( 2), 103a-103a.
Sartoris, S. Testi, M. G. Stefani, E. Chignola, R. Guerriero, C. Matucci, A. Cestari, T. Scarpa, A. Riviera, A. P. Zanoni, G. Tridente, G. Andrighetto, G. , Induction of an antitumour adaptive immune response elicited by tumour cells expressing de novo B7-1 mainly depends on the anatomical site of their delivery: the dose applied regulates the expansion of the response, Immunology, 2003, 110 (4), 474-81.
Madonini, E. Agostinis, F. Barra, R. Berra, A. Donadio, D. Pappacoda, A. Stefani, E. Tierno, E. , Long-term and preventive effects of sublingual allergen-specific immunotherapy: a retrospective, multicentric study, Int J Immunopathol Pharmacol, 2003, 16 (1), 73-9.
Guerriero, C. Zoccatelli, G. Stefani, E. Sartoris, S. Cestari, T. Riviera, A. P. Tridente, G. Andrighetto, G. Chignola, R. , Myelin basic protein epitopes secreted by human T cells encounter natural autoantibodies in the serum, J Neuroimmunol, 2003, 141 (1-2), 83-9.
Vella, A. Sartoris, S. Bambara, L. Ortolani, R. Carletto, A. Biasi, D. Stefani, E. Tridente, G. , Cell contact-dependent PMN HLA-DR and CD69 membrane expression induced by autologous mono-lymphocytes and cell lines, Inflammation, 2002, 26 (4), 143-52.
Alioua, A. Mahajan, A. Nishimaru, K. Zarei, M. M. Stefani, E. Toro, L. , Coupling of c-Src to large conductance voltage- and Ca2+-activated K+ channels as a new mechanism of agonist-induced vasoconstriction, Proceedings of the National Academy of Sciences of the United States of America, 2002, 99 ( 22), 14560-14565.
Eghbali, M. Olcese, R. Zarei, M. M. Toro, L. Stefani, E. , External pore collapse as an inactivation mechanism for Kv4.3 K+ channels, Journal of Membrane Biology, 2002, 188 ( 1), 73-86.
Mahajan, A. Alioua, A. Nishimaru, K. Zarei, M. M. Stefani, E. Toro, L. , Functional coupling of MaxiK channels with tyrosine kinase (c-Src) mediates human coronary vasorelaxation, Biophysical Journal, 2002, 82 ( 1), 247a-247a.
Helguera, G. Olcese, R. Song, M. Toro, L. Stefani, E. , Tissue-specific regulation of Ca2+ channel protein expression by sex hormones, Biochimica Et Biophysica Acta-General Subjects, 2002, 1569 ( 1-3), 59-66.
Olcese, R. Sigg, D. Latorre, R. Bezanilla, F. Stefani, E. , A conducting state with properties of a slow inactivated state in a Shaker K+ channel mutant, Journal of General Physiology, 2001, 117 ( 2), 149-163.
Zarei, M. M. Zhu, N. Alioua, A. Eghbali, M. Stefani, E. Toro, L. , A novel MaxiK splice variant exhibits dominant-negative properties for surface expression, Journal of Biological Chemistry, 2001, 276 ( 19), 16232-16239.
Zhang, Z. M. Tang, J. S. Tikunova, S. Johnson, J. D. Chen, Z. G. Qin, N. Dietrich, A. Stefani, E. Birnbaumer, L. Zhu, M. X. , Activation of Trp3 by inositol 1,4,5-trisphosphate receptors through displacement of inhibitory calmodulin from a common binding domain, Proceedings of the National Academy of Sciences of the United States of America, 2001, 98 ( 6), 3168-3173.
Marijic, J. Li, Q. X. Song, M. Nishimaru, K. Stefani, E. Toro, L. , Decreased expression of voltage- and Ca2+-activated K+ channels in coronary smooth muscle during aging, Circulation Research, 2001, 88 ( 2), 210-216.
Song, M. Helguera, G. Eghbali, M. Zhu, N. Zarei, M. M. Olcese, R. Toro, L. Stefani, E. , Remodeling of Kv4.3 potassium channel gene expression under the control of sex hormones, Journal of Biological Chemistry, 2001, 276 ( 34), 31883-31890.
Sigg, D. Bezanilla, F. Stefani, E. , Temperature dependence of the early component of gating currents from Shaker K channels, Biophysical Journal, 2001, 80 ( 1), 219a-219a.
Olcese, R. Sigg, D. M. Latorre, R. Stefani, E. Bezanilla, F. , A conducting slow inactivated Shaker channel?, Biophysical Journal, 2000, 78 ( 1), 214a-214a.
Li, Q. Marijic, J. Song, M. Stefani, E. Toro, L. , Change of Matik channel density in coronary artery with aging, Biophysical Journal, 2000, 78 ( 1), 93a-93a.
Song, M. Zhu, N. Eghbali, M. Toro, L. Stefani, E. , Changes of expression levels of Kv4.3 channels during pregnancy., Biophysical Journal, 2000, 78 ( 1), 449a-449a.
Zarei, M. M. Tora, L. Stefani, E. , Characterization of Ca2+ channel subtypes in the supraoptic nucleus., Biophysical Journal, 2000, 78 ( 1), 199a-199a.
Stefani, E. Sigg, D. Bezanilla, F. , Correlation between the early component of gating current and total gating current in shaker K channels, Biophysical Journal, 2000, 78 ( 1), 7A-7A.
Platano, D. Qin, N. Noceti, F. Birnbaumer, L. Stefani, E. Olcese, R. , Expression of the alpha(2)delta subunit interferes with prepulse facilitation in cardiac L-type calcium channefs, Biophysical Journal, 2000, 78 ( 6), 2959-2972.
Olcese, R. Toro, L. Stefani, E. , Reduction of single channel conductance by D to N mutation in the hSlo potassium channel pore, Biophysical Journal, 2000, 78 ( 1), 93a-93a.
Song, M. Barila, B. Olcese, R. Zhu, N. Toro, L. Stefani, E. , Regulation of MaxiK channel expression during pregnancy, Journal of Physiology-London, 2000, 523 (number), 51s-52s.
Madonini, E. Agostinis, F. Barra, R. Berra, A. Donadio, D. Stefani, E. Pappacoda, A. Tierno, E. , Safety and efficacy evaluation of sublingual allergen-specific immunotherapy - A retrospective, multicenter study, International Journal of Immunopathology and Pharmacology, 2000, 13 ( 2), 77-81.
Helguera, G. Song, M. Olcese, R. Toro, L. Stefani, E. , Tissue specific changes in expression levels of Ca2+ and K+ channels during pregnancy, Biophysical Journal, 2000, 78 ( 1), 350a-350a.
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