California NanoSystems Institute
CNSI
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Shimon Weiss, Sc.D.

   
Dean M. Willard Chair, Chemistry and Biochemistry
Professor, Chemistry and Biochemistry, Physiology
Director, Advanced Light Microscopy/Spectroscopy Lab
Member, California NanoSystems Institute

Education:
Degrees:
Sc.D., Israel Institute of Technology, 1989
B.S., Technion, Israel Institute of Technology, 1984

Honors and Awards:
2005 Royal College of Physicians, London, Rank Prize
2001 Michael and Kate Barany, Biophysical Society Award
1999 Optical Society of America, Fellow
1993 The Director, Outstanding Performance Award, Lawrence Berkeley Laboratory

Certifications:
Professional Societies:
AAAS, AAAS
ACS, ACS
APS, APS
Biophysical Society , Biophysical Society
Fellow, Optical Society of America (1999)

Education:
Fellowship:
1989 - 1990 Rothschild Fellowship, Rothschild Fellowship

Contact Information:
Work Email Address: sweiss@chem.ucla.edu
Laboratory Address: Young Hal 2002 & 2041
Work Address: Young Hall 2037
Home Page: http://www.chem.ucla.edu/dept/Faculty/sweiss/
Home Page: http://www.physiology.ucla.edu/faculty/sweiss.htm
Laboratory: http://smb.chem.ucla.edu/
ARR Papers: http://www.cnsi.ucla.edu/arr/personnel-papers?personnel_id=46200
Lab Number: 1 (310) 794-7158 Lab
1 (310) 794-7271 Lab
Work Phone Number: 1 (310) 794-0093 Office
Technical Research Interest:

Biophysics, Biophysical Chemistry, Nano-Spectroscopy, Bio- Nano-Technology. Single molecule detection and spectroscopy, application of single molecule detection to biology, dynamic structural/molecular biology, protein folding, protein-protein and protein-DNA interactions, single molecule enzymology, novel Bio-Nano-Technology probes, semiconductor nanocrystals, quantum dots, fluorescence microscopy/spectroscopy, physics/chemistry of mesoscopic systems, scanning probe microscopies, near-field microscopy, ultrafast processes in mesoscopic systems, ultrafast processes on surfaces.



Selected Publications:

Gassman, N. R. Ho, S. O. Korlann, Y. Chiang, J. Wu, Y. Perry, L. J. Kim, Y. Weiss, S. , In vivo assembly and single-molecule characterization of the transcription machinery from Shewanella oneidensis MR-1, Protein Expr Purif, 2009, 65 (1), 66-76.
Ebenstein, Y. Gassman, N. Kim, S. Antelman, J. Kim, Y. Ho, S. Samuel, R. Michalet, X. Weiss, S. , Lighting up individual DNA binding proteins with quantum dots, Nano Lett, 2009, 9 (4), 1598-603.
Antelman, J. Wilking-Chang, C. Weiss, S. Michalet, X. , Nanometer distance measurements between multicolor quantum dots, Nano Lett, 2009, 9 (5), 2199-205.
Bentolila, L. A. Ebenstein, Y. Weiss, S. , Quantum dots for in vivo small-animal imaging, J Nucl Med, 2009, 50 (4), 493-6.
Kim, Y.; Ho, S.O.; Gassman, N.R.; Korlann, Y.; Landrof, E.V.; Collart, F.; Weiss, S., Efficient Site-Specific Labeling of Proteins Via Cysteines, Bioconjugate Chemistry, 2008, 19 (3), 786-791.
Iyar, G.; Michalet, X.; Pinaud, F.F.; Matyas, C.E.; Chang, Y.P.; Payne, G.; Weiss, S., High Affinity scFv-hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells, Nano Letters, 2008, 8 (12), 4618-4623.
Korlann, Y.; Dertinger, T.; Michalet, X.; Weiss, S.; Enderlein, J., Measuring Diffusion with Polarization-modulation Dual-focus Fluorescence Correlation Spectroscopy, Optics Express, 2008, 16, 14609-14616.
Hamadani, K.M.; Weiss, S., Non-equilibrium Single Molecule Protein Folding in a Co-axial Mixer, Biophysical Journal, 2008, 95, 352-365.
Schipper, M.L.; Iyer, G.; Koh, A.L.; Cheng, Z.; Ebenstein, Y.; Aharoni, A.; Keren, S.; Bentolila, L.A.; Li, J.; Rao, J.; Chen, X.; Banin, U.; Wu, A.M.; Sinclair, R.; Weiss, S.; Gambhir, S.S., Particle Size, Surface Coating, and Pegylation Influence the Biodistribution of Quantum Dots in Living Mice, Small, 2008, Internet Publication.
Chang, Y.P.; Pinaud, F.; Michalet, X.; Iyer, G.; Weiss, S., Tracking Bio-molecules in Live Cells Using Quantum Dots, Journal of Biophotonics, 2008, 4, 287-298.
Schipper, M.L.; Cheng, Z.; Lee, S.W.; Keren, S.; Bentolila, L.A.; Sundaresan, G.; Iyer, G.; Gheysens,O.; Ebenstein, Y.; Li, J.; Rao, J.; Chen, X.; Wu, A.M.; Weiss, S.; Gambhir, S.S., MicroPET-Based Biodistribution of Quantum Dots in Living Mice, J. of Nuclear Medicine, 2007 (48), 1511-1518.
Doose, S.; Heilemann, M.; Michalet, X.; Weiss, S.; and Kapanidis A.N., Periodic Acceptor Excitation Spectroscopy of Single Molecules, European Biophysical J., 2007, 36, 669-674.
Kong, X.; Nir, E.; Hamadani, K.; Weiss, S., Photobleaching Pathways in Single Molecule FRET Experiments, J. Am. Chem. Soc., 2007, 129, 4643-4654.
Majumdar, D.S.; Smirnova, I.; Kasho, V.; Nir, E.; Kong, X.; Weiss, S.; Kaback, H.R., Single-molecule FRET Reveals a Sugar-Induced Conformational Dynamics in LacY, Proc Natl Acad Sci USA, 2007, 104 (31), 12640-12645.
Tsay, J.M.; Trzoss, M.; Shi, L.; Komg, X.; Selke, M.; Jung, M.E.; Weiss, S., Singlet Oxygen Production by Peptide-coated Quantum Dot-Photosensitizer Conjugates, J. Am. Chem. Soc., 2007, 129 (21), 6865-6871.
Iyer, G.; Pinaud, F.; Tsay, J.M.; and Weiss, S., Solubilization of Quantum Dots with a Recombinant Peptide Expressed in E.coli, Small, 2007, 3, 793-798.
Lee, N.K.; Kapanidis, A.N.; Koh, H.R.; Korlann, Y.; Ho, S.O.; Kim; Y.;Gassman, N.; Kim, S.K.; and Weiss, S., Three-color alternating-laser excitation of single molecules: simultaneous monitoring of multiple interactions and distances, Biophysical Journal, 2007, 92, 303-12.
Pinaud F, Michalet X, Tsay JM, Doose S, Li JJ, Iyer G, Weiss S, Advances in fluorescence imaging with quantum dot bio-probes, Biomaterials, 2006, 27 (9), 1679-87.
Michalet X, Siegmund OHW, Vallerga JV, JelinskyP, Millaud JE and Weiss S, Detectors for ingle -molecule fluorescence imaging and spectroscopy, Journal of Modern Optics, 2006, in press.
Kapanidis, A.N.; Margeat, E.; Ho, S.O.; Kortkhonjia, E.; Weiss, S.; and Ebright, R.H., Initial transcription by RNA polymerase proceeds through a DNA-scrunching mechanism, Science, 2006, 314, 1144-1147.
Iyer, G. Pinaud, F. Tsay, J. Li, J. J. Bentolila, L. A. Michalet, X. Weiss, S. , Peptide coated quantum dots for biological applications, IEEE Trans Nanobioscience, 2006, 5 (4), 231-8.
Iyer, G.; Pinaud, F.; Tsay, J.M.; Li, J.J.; Bentolila, L.A.; Michalet, X.; and Weiss, S., Peptide coated quantum dots for biological applications, IEEE Transactions on Nanobioscience, 2006, 5, 231-8.
Michalet X, Siegmund OHW, Vallerga JV, Jelinsky P, Millaud JE, Weiss S, Photon-Counting H33D Detector for Biological Fluorescence Imaging, Nuclear Instruments and Methods in Physics Research, 2006 (567), 133.
Tsay JM, Doose S and Weiss S, Rotational and Translational Diffusion of CdSe/CdS/ZnS Nanorods Studied by Fluorescence Correlation Spectroscopy, J. Am. Chem. Soc., 2006, 128 (5), 1639.
Nir, E.; Michalet, X.; Hamadani. K.; Laurence, T.A.; Neuhauser, D.; Kovchegov, Y.; and Weiss, S., Shot-noise limited single-molecule FRET histogram: comparison between theory and experiments, J. Phys. Chem. B., 2006, 110, 22103-22124.
Michalet X, Weiss S, Jger M, Single-molecule Fluorescence Studies of Protein Folding and Conformational Dynamics, Chemical Reviews, , 2006, 106 (5), 1785-813.
Jager M, Nir E, Weiss S, Site-specific labeling of proteins for single molecule FRET by combining chemical and enzymatic modification, Protein Science, 2006, 15, 640-646.
Lee, NK Kapanidis, AN Wang, Y Michalet, X Mukhopadhyay, J Ebright, RH Weiss, S, Accurate FRET measurements within single diffusing biomolecules using alternating-laser excitation., Biophysical journal. , 2005, 88 (4), 2939-53.
Kapanidis AN, Laurence TA, Lee NK, Margeat E, Kong X, Weiss S, Alternating-laser excitation of single molecules., Accounts of chemical research. , 2005, 38 (7), 523-33.
Doose S, Tsay JM, Pinaud F, Weiss S, Comparison of photophysical and colloidal properties of biocompatible semiconductor nanocrystals using fluorescence correlation spectroscopy., Analytical chemistry. , 2005, 77 (7), 2235-42.
Margeat E, Kapanidis AN, Tinnefeld P, Wang Y, Mukhopadhyay J, Ebright RH and Weiss S, Direct observation of abortive initiation and promoter escape within single immoblized transcription complexes, Biophys J., 2005, 90, 1419-1431.
Tsay, Jm.;, Doose, S.; Pinaud, F.; Weiss, S.;, Enhancing the Photoluminescence of Peptide-coated Nanocrystals, Proceedings of SPIE on Progress on Biomedical Optics and imaging:, Nanobiophotonics and Biomedical Applications II, 2005, 6 (20), 139-147.
Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, Li JJ, Iyer G, Weiss S, Peptide-coated semiconductor nanocrystals for biomedical applications, Proceedings of SPIE, 2005, 5704, 57-68.
Laurence TA, Kong X, Jager M, Weiss S, Probing structural heterogeneities and fluctuations of nucleic acids and denatured proteins, Proc Natl Acad Sci U S A., 2005, 102 (48), 17348-53 .
Jager M, Michalet X, Weiss S, Protein-protein interactions as a tool for site specific labeling of proteins, Protein Science, 2005, 14, 2059-2068.
Michalet X, Pinaud F, Bentolila LA, Tsay J, Doose S, Li J, Sundaresan G, Wu AM, Gambhir SS, Weiss S, Quantum Dots for Live Cells and in vivo Imaging, Diagnostics and Beyond, Science , 2005, 307 (5709), 538-544.
Kapanidis AN, Margeat E, Laurence TA, Doose S, Ho, SO, Kortkhonjia ME, Mekler V, Ebright RH and Weiss S, Retention of transcription initation factor ??70 in transcription elongation: single molecule analysis, Molecular Cell, 2005, 20 (3), 347-56.
Lee NK, Kapanidis AN, Michalet X, Mukhophadyay J, Ebright RH, Weiss S, Towards structure from single biomolecules: accurate FRET through alternating-laser excitation, Biophys J, 2005, 88 (4), 2939-2953.
Li JJ, Tsay J, Michalet X, Weiss S, Wave-function engineering: from quantum wells to near-infrared type-II colloidal quantum dots synthesized by layer-by-layer colloidal epitaxy, Chemical Physics (Special Issue on Molecular Nanoscience-In Honor of Daniel S. Chemla on his 65th Birthday), 2005, 318, 82-90.
Baer, R.; Neuhauser, D.; Weiss, S., Enhanced Absorption Induced by a Metallic Nanoshell, Nano Letters, 2004, 4 (1), 85-88.
Kapanidis AN,Lee NK, Laurence TA, Doose S, Margeat E, Weiss S, Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules., Proceedings of the National Academy of Sciences of the United States of America. , 2004, 101 (24), 8936-41.
Michalet X, Kapanidis AN, Laurence T, Pinaud F, Doose S, Pflughoefft M, Weiss S, The power and prospects of fluorescence microscopies and spectroscopies., Annual review of biophysics and biomolecular structure. , 2003, 32, 161-82.